UH Faculty, Staff, and Student Works
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Browsing UH Faculty, Staff, and Student Works by Subject "16S rRNA"
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Item Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces(Microbiome, 4/8/2019) Sielaff, Aleksandra Checinska; Urbaniak, Camilla; Mohan, Ganesh Babu Malli; Stepanov, Victor G.; Tran, Quyen; Wood, Jason M.; Minich, Jeremiah; McDonald, Daniel; Mayer, Teresa; Knight, Rob; Karouia, Fathi; Fox, George E.; Venkateswaran, KasthuriThe International Space Station (ISS) is a closed system inhabited by microorganisms originating from life support systems, cargo, and crew that are exposed to unique selective pressures such as microgravity. To date, mandatory microbial monitoring and observational studies of spacecraft and space stations have been conducted by traditional culture methods, although it is known that many microbes cannot be cultured with standard techniques. To fully appreciate the true number and diversity of microbes that survive in the ISS, molecular and culture-based methods were used to assess microbial communities on ISS surfaces. Samples were taken at eight pre-defined locations during three flight missions spanning 14 months and analyzed upon return to Earth.