Studies on erythrocyte sugar transport
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Abstract
Sugar transport was studied. In bovine and human erythrocytes. It was concluded that the bovine RBC possesses a facilitated diffusion system for the transport of various sugars, based on the demonstration of saturation kinetics and competitive inhibition. However, sugar transport In the bovine RBC, as compared to the human RBC, Is characterized by (1) a slow rate of transport, (2) lack of substrate alteration of carrier mobility, and (3) insensitivity to protein sulfhydryl reagents. Membrane components of the human RBC sugar transport system were Identified by dual Isotopic, differential labeling with 2-fluoro-2,4-dinitrobenzene (DNFB). The labeling technique was based on the ability of high affinity substrates to Induce conformational changes In the transport mechanism, thereby. Increasing Its incorporation of DNFB. The results indicate that, under these conditions, the site of DNFB incorporation is the membranephospholipids, rather than membrane proteln(s). Thus It appears that membrane phospholipids are an integral part of the sugar transport system.