Isolation, cloning and sequencing of a putative petD gene from Agmenellum quadruplicatum PR-6

Southern blots containing genomic Agmenellum quadruplicatum DNA digested with various restriction enzymes were screened for the petD gene using 1) a radiolabelled RNA probe from the petD gene from spinach and 2) a 21 base long end labelled oligomer with 32 fold degeneracy synthesized according to a highly conserved area of the subunit IV gene. The RNA probe did not hybridize to the blots apparently indicating not enough sequence similarity between the petD genes from the two sources. [...]