Gas chromatography and mass spectrometry of N-trifluoro-acetyl amino acid isopropyl esters

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dc.contributor.advisorOró, John F.
dc.contributor.committeeMemberZlatkis, Albert
dc.contributor.committeeMemberFlory, Donald A.
dc.contributor.committeeMemberParr, Wolfgang
dc.contributor.committeeMemberKimball, Aubrey P.
dc.contributor.committeeMemberBartel, Allen H.
dc.creatorSeiler, Achim Heinrich
dc.date.accessioned2022-06-20T16:57:57Z
dc.date.available2022-06-20T16:57:57Z
dc.date.issued1972
dc.description.abstractAll primary α-amino acids detected in the Murchison Meteorite were also found in Miller's recent prebiotic synthesis of amino acids by electric discharge. A procedure was established that allows the separation and ready identification of these amino acids by gas chromatography-mass spectrometry. The retention times of the amino acids as their N-trifluoro-acetyl (N-TFA) isopropyl ester derivatives were determined in methylene units in comparison with a standard of aliphatic hydrocarbons on a SF-96 stainless steel needle stock. The mass spectra of the derivatized amino acids were taken and standardized. These standards have been recorded on magnetic tape and will be used for the computerized identification of mass spectra of N-TFA amino acid isopropyl esters from extraterrestrial or prebiotic samples to be investigated. The applicability of the method was tested on samples from a polymerization experiment of hydrogen cyanide received from J. P. Ferris. Five protein and two nonprotein amino acids were isolated, separated, and identified by gas chromatography in combination with mass spectrometry.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other23164629
dc.identifier.urihttps://hdl.handle.net/10657/9504
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleGas chromatography and mass spectrometry of N-trifluoro-acetyl amino acid isopropyl esters
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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