The purification and characterization of L-malate : NAD oxidoreductase in Physarum polycephalum

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dc.contributor.advisorHenney, Henry R., Jr.
dc.contributor.committeeMemberCowles, Joe R.
dc.contributor.committeeMemberGray, Horace B., Jr.
dc.contributor.committeeMemberAumann, Glenn David
dc.creatorTeague, William Martin
dc.date.accessioned2022-03-16T15:47:10Z
dc.date.available2022-03-16T15:47:10Z
dc.date.issued1972
dc.description.abstractTwo isoenzymes of malate dehydrogenase could be demonstrated for plasmodia of Physarum polycephalum by polyacrylamide gel electrophoresis. The more cathodal form is uniquely associated with mitochondria (M-MDH) while the other form is found in the soluble cytoplasm (S-MDH). The isoenzymes are separated by acetone fractionation of soluble plasmodial homogenates acidified to pH 5.0. The M-MDH is purified 176-fold by cetylpyridinium chloride treatment, fractionation with ammonium sulfate, and gradient elution from sulfoethyl cellulose at pH 6.0. The S-MDH is purified 123-fold by ammonium sulfate fractionation, diethylaminoethyl cellulose chromatography, and gradient elution from sulfoethyl cellulose at pH 5.5. Both isoenzymes could be further characterized by chemical and physical parameters. [...]
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other23165872
dc.identifier.urihttps://hdl.handle.net/10657/8901
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. §107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleThe purification and characterization of L-malate : NAD oxidoreductase in Physarum polycephalum
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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