A characterization of the ribonucleic acid and ribosomes of the Myxomycete Physarum rigidum

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dc.contributor.advisorHenney, Henry R., Jr.
dc.contributor.committeeMemberKimball, Aubrey P.
dc.contributor.committeeMemberBartel, Allen H.
dc.contributor.committeeMemberAumann, Glenn David
dc.creatorJungkind, Donald
dc.date.accessioned2022-07-07T20:12:22Z
dc.date.available2022-07-07T20:12:22Z
dc.date.issued1968
dc.description.abstractPlasmodium of Physarum rigidum A was grown in axenic shake culture at 24[degrees]C in a soluble semi-defined medium. Aliquots were removed at 24 hour intervals and measured for dry weight, protein and RNA content. By day 13, the maximum values were reached. These were 85.0, 51.0 and 8.3 mg, respectively, per 50 ml of media. Extracts were made in a pH 7.4 buffer containing 0.01 M Mg[raised ++] ions, and the S[degrees][lowered 20] value of the ribosomes was determined to be 79.6. The magnesium concentration was lowered and the S[lowered 20] values of the subunits were determined to be 37.6 and 57.5 respectively. The sedimentation values of phenol purified total RNA were obtained using the analytical ultracentrifuge and absorption optics. The boundaries found had S[degrees][lowered 20] values of 5.1, 17.7 and 26.8. The nucleotide composition of the total RNA and ribosomal RNA was determined on multiple samples by chromatography on AG1-X8 formate resin following alkaline hydrolysis. The total RNA content of guanylic, cytidylic, adenylic, and uridylic acids respectively is 33.67[plus-minus]0.99, 18.82[plus-minus]0.60, 24.09[plus-minus]0.70, and 23.42[plus-minus]0.36. The corresponding composition of ribosomal RNA is 31.62[plus-minus]1.18, 19.33[plus-minus]1.29, 26.25[plus-minus]0.42, and 22.80[plus-minus]0.67.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other17972110
dc.identifier.urihttps://hdl.handle.net/10657/10441
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleA characterization of the ribonucleic acid and ribosomes of the Myxomycete Physarum rigidum
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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