Expression and purification of active human receptor interacting protein 1 kinase using a baculovirus system



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Protein Expression and Purification


Receptor Interacting Protein 1 (RIP1) kinase is one of the key mediators of tumor necrosis factor alpha (TNF-?) signaling and is critical for activation of necroptotic cell death. We developed a method for expression of recombinant kinase, utilizing baculovirus co-infection of Cdc37, an Hsp90 co-chaperone, and RIP1-His, followed by a two-step purification scheme. After optimization, 1–3 mg of highly purified RIP1 kinase was typically obtained from a 1 L of Sf9 cells. The recombinant protein displayed kinase activity that was blocked by RIP1 inhibitors, necrostatins. The purified protein was used to develop a simple and robust thermal shift assay for further assessment of RIP1 inhibitors.



RIP1 kinase, Cdc37, Necrostatins, Baculovirus, Thermal shift assay


Copyright 2013 Protein Expression and Purification. This is a post-print version of a published paper that is available at: Recommended citation: Maki, Jenny L., J. Tres Brazell, Xin Teng, Gregory D. Cuny, and Alexei Degterev. "Expression and purification of active receptor interacting protein 1 kinase using a baculovirus system." Protein expression and purification 89, no. 2 (2013): 156-161.doi: 10.1016/j.pep.2013.03.002. This item has been deposited in accordance with publisher copyright and licensing terms and with the author's permission.