Design, Optimization, and Validation of Multiplex Immunofluorescence Assay for Detecting Biomarker Expression on Circulating Tumor Cells in Breast Cancer

The purpose of this study was to design, optimize and validate a multiplex immunofluorescence (IF) assay to identify CTCsanddetect apanel of biomarkers onCTCs. This project was completed with contributions from Lacey E. Dobrolecki and Michael T. Lewis from Baylor College of Medicine.