Biological activity and biochemical mechanisms of action studies of [upsilon]-ox and thymox in cultured L1210 cells

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dc.contributor.advisorKimball, Aubrey P.
dc.contributor.committeeMemberBartel, Allen H.
dc.contributor.committeeMemberOro, John F.
dc.contributor.committeeMemberBear, John L.
dc.creatorLewis, Marian L.
dc.date.accessioned2024-03-27T20:55:14Z
dc.date.available2024-03-27T20:55:14Z
dc.date.issued1979
dc.description.abstractThymox and Vox induced time and concentration dependent cytotoxic and cytostatic effects in LI210 cells. Affected cells were irreversibly blocked in the G2 phase of the cell cycle. Minimum effective exposure time of cells to Vox and thymox was 30 minutes and 2 hours respectively. In synchronized cells, both compounds were cytotoxic in all cell cycle phases, but exhibited greatest effect in late Gl-early S and in G2. Biochemical mechanisms of action included inhibition of ONA more than RNA synthesis. Activity of DNA and RNA polymerases in crude cellular extracts were reduced. Protein synthesis was inhibited, but some protein and RNA synthesis appeared to continue in G2 blocked cells. Microtubule protein levels, detected by the colchicine binding assay, were greatly reduced. Preliminary evidence suggests that Vox and thymox inhibited the synthesis of microtubule protein. This may account in part for the arrest of cells in the G2 phase of the cell cycle.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other6771953
dc.identifier.urihttps://hdl.handle.net/10657/16714
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleBiological activity and biochemical mechanisms of action studies of [upsilon]-ox and thymox in cultured L1210 cells
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiophysical Sciences, Department of
thesis.degree.disciplineBiophysical Sciences
thesis.degree.grantorUniversity of Houston

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