Flotation Immunoassay: Masking the Signal from Free Reporters in Sandwich Immunoassays


In this work, we demonstrate that signal-masking reagents together with appropriate capture antibody carriers can eliminate the washing steps in sandwich immunoassays. A flotation immunoassay (FI) platform was developed with horseradish peroxidase chemiluminescence as the reporter system, the dye Brilliant Blue FCF as the signal-masking reagent, and buoyant silica micro-bubbles as the capture antibody carriers. Only reporters captured on micro-bubbles float above the dye and become visible in an analyte-dependent manner. These FIs are capable of detecting proteins down to attomole levels and as few as 106 virus particles. This signal-masking strategy represents a novel approach to simple, sensitive and quantitative immunoassays in both laboratory and point-of-care settings.



Biochemical assays, High-throughput screening


Copyright 2016 Scientific Reports. Recommended citation:Chen, Hui, Anna EV Hagstr鰉, Jinsu Kim, Gavin Garvey, Andrew Paterson, Federico Ruiz-Ruiz, Balakrishnan Raja et al. "Flotation immunoassay: Masking the signal from free reporters in sandwich immunoassays." Scientific reports 6 (2016): 24297.doi:10.1038/srep24297. URL: https://www.nature.com/articles/srep24297. Reproduced in accordance with the original publisher's licensing terms and with permission from the authors.