Sensitive Detection of Norovirus Using Phage Nanoparticle Reporters in Lateral-Flow Assay


Noroviruses are recognized worldwide as the principal cause of acute, non-bacterial gastroenteritis, resulting in 19-21 million cases of disease every year in the United States. Noroviruses have a very low infectious dose, a short incubation period, high resistance to traditional disinfection techniques and multiple modes of transmission, making early, point-of-care detection essential for controlling the spread of the disease. The traditional diagnostic tools, electron microscopy, RT-PCR and ELISA require sophisticated and expensive instrumentation, and are considered too laborious and slow to be useful during severe outbreaks. In this paper we describe the development of a new, rapid and sensitive lateral-flow assay using labeled phage particles for the detection of the prototypical norovirus GI.1 (Norwalk), with a limit of detection of 107 virus-like particles per mL, one hundred-fold lower than a conventional gold nanoparticle lateral-flow assay using the same antibody pair.




Copyright 2015 PLoS ONE. Recommended citation: Hagstr鰉, Anna EV, Gavin Garvey, Andrew S. Paterson, Sagar Dhamane, Meena Adhikari, Mary K. Estes, Ulrich Strych, Katerina Kourentzi, Robert L. Atmar, and Richard C. Willson. "Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay." PloS one 10, no. 5 (2015): e0126571. DOI:10.1371/journal.pone.0126571. URL: Reproduced in accordance with the original publisher's licensing terms and with permission from the authors.