Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation

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dc.contributor.authorLitvinov, Julia
dc.contributor.authorHagstrom, Anna E. V.
dc.contributor.authorLopez, Yubitza
dc.contributor.authorAdhikari, Meenu
dc.contributor.authorKourentzi, Katerina D.
dc.contributor.authorStrych, Ulrich
dc.contributor.authorMonzon, Federico A.
dc.contributor.authorFoster, William
dc.contributor.authorCagle, Philip T.
dc.contributor.authorWillson, Richard C.
dc.date.accessioned2020-03-11T17:16:24Z
dc.date.available2020-03-11T17:16:24Z
dc.date.issued2015-09
dc.description.abstractWe report a novel, modular approach to immuno-detection based on antibody recognition and PCR read-out that employs antibody-conjugated bacteriophage, easily-manipulated nonpathogenic viruses, as affinity agents. Our platform employs phage genetically tagged for in vivo biotinylation during phage maturation that can easily be linked, through avidin, to any biotinylatable affinity agent, including full-length antibodies, peptides, lectins or aptamers. The presence of analyte is reported with high sensitivity through real-time PCR. This approach avoids the need to clone antibody-encoding DNA fragments, allows the use of full-length, high affinity antibodies and, by having DNA reporters naturally encapsulated inside the bacteriophage, greatly reduces nonspecific binding of DNA. We validate the efficacy of this new approach through the detection of VEGF (Vascular Endothelial Growth Factor), a known angiogenic cancer biomarker protein, at attomolar concentrations in bronchoalveolar lavage (BAL) fluid.
dc.identifier.citationCopyright 2014 Biotechonology Letters. This is a post-print version of a published paper that is available at: https://link.springer.com/article/10.1007/s10529-014-1555-9. Recommended citation: Litvinov, Julia, Anna EV Hagstr鰉, Yubitza Lopez, Meenu Adhikari, Katerina Kourentzi, Ulrich Strych, Federico A. Monzon, William Foster, Philip T. Cagle, and Richard C. Willson. "Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation." Biotechnology letters 36, no. 9 (2014): 1863-1868. DOI: 10.1007/s10529-014-1555-9. This item has been deposited in accordance with the publisher copyright and licensing terms and with the author's permission.
dc.identifier.urihttps://hdl.handle.net/10657/6215
dc.language.isoen_US
dc.publisherBiotechnology Letters
dc.subjectBacteriophage
dc.subjectbiomarker
dc.subjectELISA
dc.subjectimmuno-PCR
dc.subjectimmuno-phage assay
dc.titleUltrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation
dc.typeArticle

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