Binding of small organic molecules to a model nucleoprotein

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dc.contributor.advisorBecker, Ralph S.
dc.contributor.committeeMemberBartel, Allen H.
dc.contributor.committeeMemberOró, John F.
dc.contributor.committeeMemberCollins, R. Eugene
dc.contributor.committeeMemberWentworth, Wayne E.
dc.creatorHornish, Dale Martin
dc.date.accessioned2022-08-10T21:22:37Z
dc.date.available2022-08-10T21:22:37Z
dc.date.copyright1966
dc.date.issued1968
dc.description.abstractThe importance of DNA-protein-small molecule interactions in the control of genetic expression is now clearly established. Spectrophotometric and fluorometric titration of acridine orange, 9-aminoacridine, and ethidium bromide with DNA and a model nucleoprotein, composed of DNA complexed with poly-L-lysine, indicate that these small molecules successfully compete with poly-L-lysine for binding sites on DNA. A comparison of the absorption spectra of l-hydroxyacridine and l-hydroxyanthracene in the presence of DNA gives some insight into the nature of acridine-DNA interaction under the conditions of our experiments. Also described are preliminary efforts toward the extension of existing techniques to the study of carcinogenic hydrocarbons in this context. The possible significance in nature of small molecule-protein competition for DNA binding sites is discussed.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other17885685
dc.identifier.urihttps://hdl.handle.net/10657/10754
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleBinding of small organic molecules to a model nucleoprotein
dc.type.dcmiText
dc.type.genreThesis
dcterms.accessRightsThe full text of this item is not available at this time because it contains documents that are presumed to be under copyright and are accessible only to users who have an active CougarNet ID. This item will continue to be made available through interlibrary loan.
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiochemical and Biophysical Sciences, Department of
thesis.degree.disciplineBiochemical and Biophysical Sciences
thesis.degree.grantorUniversity of Houston
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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