Partial purification of phenylalanine hydroxylase from dog liver

Simple item page
dc.contributor.advisorBartel, Allen H.
dc.contributor.committeeMemberGray, Horace B., Jr.
dc.contributor.committeeMemberSpring, Thomas G.
dc.contributor.committeeMemberMitchell, Barbara D.
dc.creatorRoss, Peggy L. Horner
dc.date.accessioned2022-05-19T17:44:52Z
dc.date.available2022-05-19T17:44:52Z
dc.date.issued1975
dc.description.abstractPhenylalanine hydroxylase from dog liver was purified ten fold. The physical and kinetic properties of phenylalanine hydroxylase from dog liver in many respects closely resemble physical and kinetic properties of the same enzyme from other organism reported in the literature. The Km value determined for phenylalanine hydroxylase from dog liver for phenylalanine is 3.7 [plus-minus] 1.1 mN; for 6,7-dimethyltetrahydropterin is 0.17 [plus-minus] 0.10 mM; and for tetrahydrobiopterin is 0.34 [plus-minus] 0.03 mH. A new assay developed in this study is especially suited for detection of phenylalanine hydroxylase from dog liver and it makes possible the detection of enzyme activity at low concentration of enzyme.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other2752390
dc.identifier.urihttps://hdl.handle.net/10657/9128
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. §107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titlePartial purification of phenylalanine hydroxylase from dog liver
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiophysical Sciences, Department of
thesis.degree.disciplineBiophysical Sciences
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ross_1975_2752390.pdf
Size:
2.21 MB
Format:
Adobe Portable Document Format
Download

Collections

Legacy Theses and Dissertations (1940-2009)