The application of gas chromatographic techniques in the determination of monosaccharides, oligosaccharides and volatiles



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A concentration technique for the analysis of mono- and oligosaccharides by gas chromatography has been developed. The method increases the detection limit up to 500 times by using a double phase extraction of silylated derivatives and a splitless injection. The method has been found to be fast and reproducible for a variety of samples. The determination included liquid, solid and semisolid samples. Some samples of biological origin as cell cultures and yeasts were determined. The method was found particularly useful in the taxonomic classification of four strains of yeast. Headspace sampling was applied to the analysis of volatiles from several yeast strains and the results compared. Some differences in the profiles were found. The transevaporation technique was applied to the analysis of volatiles from cell cultures.