Studies on membrane-bound ATPase activity from soybean cells

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dc.contributor.advisorHendrix, Donald L.
dc.contributor.committeeMemberCowles, Joe R.
dc.contributor.committeeMemberSpring, Thomas G.
dc.creatorKennedy, Ralph Michael
dc.date.accessioned2022-06-21T17:09:35Z
dc.date.available2022-06-21T17:09:35Z
dc.date.issued1977
dc.description.abstractA membrane fraction enriched with K^+ -stimulated ATPase and glucan synthetase activity was isolated from soybean cells. This fraction is thought to be enriched with plasma membrane. The ATPase associated with this isolated membrane fraction requires 3 mM Mg^2+ and 3 mM ATP for maximal activity. Maximal K^+ -stimulation of the enzyme occurs at 50 mM KCl in the presence of 3 mM Mg^2+ and 3 mM ATP. A pH of 6.5 was found to be optimal for ATPase activity in the presence of 50 mM KCl and slightly higher without KCl. A 1:1 ratio of Mg^2+ to ATP was found to be optimal for enzyme activity. No clear patterns of monovalent cation or anion stimulation were found, nor were any synergistic monovalent ion stimulations detectable. Only slight variations exist between membrane preparations from soybean callus and root.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other3828797
dc.identifier.urihttps://hdl.handle.net/10657/9589
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.subjectBiology
dc.subjectBiochemistry
dc.subjectATPase
dc.subjectATP
dc.titleStudies on membrane-bound ATPase activity from soybean cells
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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