The partial purification and characterization of soluble and mitochondrial malate dehydrogenase from the white shrimp (Penaeus setiferus)

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dc.contributor.advisorBartel, A. H.
dc.contributor.advisorEvans, John E.
dc.creatorEvans, Joseph Edwin
dc.date.accessioned2022-07-07T20:12:32Z
dc.date.available2022-07-07T20:12:32Z
dc.date.issued1971
dc.description.abstractMalate dehydrogenase was extracted from the muscle tissue of the White shrimp, Penaeus setiferus, and partially purified by successive ammonium sulfate fractionation and negative adsorption on carboxymethyl cellulose. The enzyme was separated into the soluble and mitochondrial form by diethylaminoethyl cellulose chromatography. Soluble and mitochondrial malate dehydrogenase were then characterized by a variety of physical-chemical and catalytic tests. Some of these tests included: polyacrylamide gel electrophoresis, Michaelis constants, NAD analogue ratios, substrate inhibition, thermostability, and molecular weight determinations. The results of these tests indicate that White shrimp malate dehydrogenase is in many ways similar to the malate dehydrogenases isolated from other organisms but differs markedly in respect to molecular weight and inhibition by oxalacetate and malate.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other17971930
dc.identifier.urihttps://hdl.handle.net/10657/10466
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleThe partial purification and characterization of soluble and mitochondrial malate dehydrogenase from the white shrimp (Penaeus setiferus)
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Arts and Sciences
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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