Screening Method for Peptoid-Based One-Bead-One-Compound (OBOC) Libraries and Analysis of a Peptoid "Hit"

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dc.contributor.advisorMohan, Chandra
dc.contributor.committeeMemberUdugamasooriya, D. Gomika
dc.contributor.committeeMemberWu, Tianfu
dc.creatorChen, Yi-Jun
dc.date.accessioned2020-01-04T01:58:33Z
dc.date.createdMay 2019
dc.date.issued2019-05
dc.date.submittedMay 2019
dc.date.updated2020-01-04T01:58:34Z
dc.description.abstractDiscovery of therapeutic or biotechnological agents is crucial in aiding the research of disease therapy. This thesis entails two projects, the first project focuses on utilizing a magnetic screening-based assay to identify peptoid-based compounds from a one-bead-one-compound (OBOC) library with specificity to human C-reactive protein, four initial hits were found, which was then repeated with a quantum dot assay that did not confirm binding of the hits to CRP due to technical concerns. The second project takes a peptoid-based hit GUCM-1 discovered from a previous screening assay with specificity for toll-interleukin 1 receptor (TIR) domain containing adaptor protein (TIRAP), an adaptor protein with a crucial role in the toll-like receptor 4 (TLR4) signal transduction pathway and studied it for its functional effects on a human myeloid cell line, results were inconclusive and further studies are needed to validate these findings.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/10657/5741
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectPeptoid
dc.subjectOne-bead-one-compound (OBOC)
dc.subjectScreening
dc.subjectTLR4
dc.subjectTIRAP
dc.subjectToll-like receptors
dc.subjectMAL
dc.titleScreening Method for Peptoid-Based One-Bead-One-Compound (OBOC) Libraries and Analysis of a Peptoid "Hit"
dc.type.dcmiText
dc.type.genreThesis
local.embargo.lift2021-05-01
local.embargo.terms2021-05-01
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science in Biomedical Engineering

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