Flow microfluorometic analysis of cellular DNA content during early stages of transformation by type C oncorna virus

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dc.contributor.advisorAllred, John C.
dc.contributor.advisorPriori, Elizabeth S.
dc.contributor.committeeMemberHungerford, Ed V., III
dc.contributor.committeeMemberJeffery, William R.
dc.creatorHilton, Donna Jean
dc.date.accessioned2022-09-23T16:46:59Z
dc.date.available2022-09-23T16:46:59Z
dc.date.copyright1977
dc.date.issued1977
dc.description.abstractFlow microfluorometric (FMF) analysis of the quantity of cell DNA is performed on normal rat kidney (NRK) cells for six days following infection with Kirsten mouse sarcoma virus (KiMSV), a type C oncorna virus. Characterization of infection of cells by morphology, immunology, growth patterns, and chromosome number reveals that KiMSV infected cells exhibit properties associated with virus transformation. FMF analysis of daily samples of noninfected and KiMSV-infected NRK cells, stained by acriflavin-Feulgen technique, indicates no significant change in ENA content of freshly infected cells from that of noninfected cells. However, NRK cells infected with KiMSV for more than 100 passages show an elevated cellular DNA content. Significance of possible artifacts arising from procedural technique for FMF data are discussed.
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other3807938
dc.identifier.urihttps://hdl.handle.net/10657/11850
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleFlow microfluorometic analysis of cellular DNA content during early stages of transformation by type C oncorna virus
dc.type.dcmiText
dc.type.genreThesis
dcterms.accessRightsThe full text of this item is not available at this time because it contains documents that are presumed to be under copyright and are accessible only to users who have an active CougarNet ID. This item will continue to be made available through interlibrary loan.
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiophysical Sciences, Department of
thesis.degree.disciplineBiophysical Sciences
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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