Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles

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dc.contributor.authorJacinto, Maria Joao
dc.contributor.authorTrabuco, Joao R. C.
dc.contributor.authorVu, Binh V.
dc.contributor.authorGarvey, Gavin
dc.contributor.authorKhodadady, Mohammad
dc.contributor.authorAzevedo, Ana M.
dc.contributor.authorAires-Barros, Maria R.
dc.contributor.authorChang, Lang
dc.contributor.authorKourentzi, Katerina D.
dc.contributor.authorLitvinov, Dmitri
dc.contributor.authorWillson, Richard C.
dc.date.accessioned2020-03-11T17:16:19Z
dc.date.available2020-03-11T17:16:19Z
dc.date.issued2018-01
dc.description.abstractLateral flow assays (LFAs) are a widely-used point-of care diagnostic format, but suffer from limited analytical sensitivity, especially when read by eye. It has recently been reported that LFA performance can be improved by using magnetic reporter particles and an external magnetic field applied at the test line. The mechanism of sensitivity/performance enhancement was suggested to be concentration/retardation of reporter particles at the test line. Here we demonstrate an additional mechanism of particle relocation where reporter particles from the lower depths of the translucent LFA strip relocate to more-visible locations nearer to the top surface, producing a more visible signal. With a magnetic field we observed an improvement in sensitivity of human chorionic gonadotropin (hCG) detection from 1.25 ng/mL to 0.31 ng/mL. We also observed an increase of the color intensity per particle in test lines when the magnetic field was present.
dc.identifier.citationCopyright 2018 PLoS ONE. Recommended citation: Jacinto, Maria Jo鉶, Jo鉶 RC Trabuco, Binh V. Vu, Gavin Garvey, Mohammad Khodadady, Ana M. Azevedo, Maria Raquel Aires-Barros et al. "Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles." PloS one 13, no. 1 (2018): e0186782. DOI: 10.1371/journal.pone.0186782. URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0186782. Reproduced in accordance with the original publisher's licensing terms and with permission from the authors.
dc.identifier.urihttps://hdl.handle.net/10657/6212
dc.language.isoen_US
dc.publisherPLoS ONE
dc.subjectMagnetic fields
dc.subjectMagnets
dc.subjectEnzyme-linked immunoassays
dc.subject3D printing
dc.subjectChorionic gonadotropin
dc.subjectElectromagnetics
dc.subjectNitrocellulose
dc.subjectVisual signals
dc.titleEnhancement of lateral flow assay performance by electromagnetic relocation of reporter particles
dc.typeArticle

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