Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy


The present work reports on in situ observations of the interaction of organic dye probe molecules and dye-labeled protein with different poly(ethylene glycol) (PEG) architectures (linear, dendron, and bottle brush). Fluorescence correlation spectroscopy (FCS) and single molecule event analysis were used to examine the nature and extent of probe朠EG interactions. The data support a sieve-like model in which size-exclusion principles determine the extent of probe朠EG interactions. Small probes are trapped by more dense PEG architectures and large probes interact more with less dense PEG surfaces. These results, and the tunable pore structure of the PEG dendrons employed in this work, suggest the viability of electrochemically-active materials for tunable surfaces.



Fluorescence correlation spectroscopy (FCS), Surface interactions, Poly(ethylene glycol) (PEG), Surface modification, Dynamics


Copyright 2011 Colloids Surf B Biointerfaces. This is a post-print version of a published paper that is available at: Recommended citation: Daniels, Charlisa R., Carmen Reznik, Rachel Kilmer, Mary Jane Felipe, Maria Celeste R. Tria, Katerina Kourentzi, Wen-Hsiang Chen, Rigoberto C. Advincula, Richard C. Willson, and Christy F. Landes. "Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy." Colloids and Surfaces B: Biointerfaces 88, no. 1 (2011): 31-38. DOI: 10.1016/j.colsurfb.2011.05.044. This item has been deposited in accordance with the publisher copyright and licensing terms and with the author's permission.