Delineation of bacterial luciferase aldehyde site by bifunctional labeling reagents and by chemical modification

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dc.contributor.advisorTu, Shiao-Chun
dc.contributor.committeeMemberCox, James R., Jr.
dc.contributor.committeeMemberFox, George E.
dc.contributor.committeeMemberEichberg, Joseph E.
dc.contributor.committeeMemberWidger, William R.
dc.creatorPaquatte, Olivier
dc.date.accessioned2023-12-13T20:53:07Z
dc.date.available2023-12-13T20:53:07Z
dc.date.issued1988
dc.description.abstractPreviously we have established that an essential cysteinyl group on the [alpha] subunit is at the aldehyde site of ([alpha beta]) dimeric Vibrio harveyi luciferase. Three isomeric bifunctional reagents have been used to further delineate the luciferase aldehyde site. These probes differ in their relative positions of and distances between the two functional groups active in chemical and photochemical labelings, respectively. Each of the probes can effectively and reversibly inactivate luciferase by forming a disulfide linkage primarily to the essential cysteinyl residue. Upon subsequent photolysis, a diazoacetate arm in each probe was activated for photochemical labeling of amino acid residues within reach. After reductive regeneration of the essential cysteinyl residue, 0.35-0.40 probe per dimeric luciferase was found to have been photochemically incorporated, correlating well with the degree of irreversible enzyme inactivation. [...]
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other20077361
dc.identifier.urihttps://hdl.handle.net/10657/15646
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.subjectAldehydes
dc.subjectPhotosensitization, Biological
dc.titleDelineation of bacterial luciferase aldehyde site by bifunctional labeling reagents and by chemical modification
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiochemical and Biophysical Sciences, Department of
thesis.degree.disciplineBiochemical and Biophysical Sciences
thesis.degree.grantorUniversity of Houston
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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