Analysis of the distribution of messenger RNA during insect development by in situ hybridization with radioactive polyuridylic acid

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dc.contributor.advisorJeffery, William R.
dc.contributor.advisorFranklin, Luther E.
dc.contributor.committeeMemberBryant, Edwin H.
dc.contributor.committeeMemberHenney, Henry R., Jr.
dc.creatorCapco, David George
dc.date.accessioned2022-06-22T18:28:00Z
dc.date.available2022-06-22T18:28:00Z
dc.date.copyright1977
dc.date.issued1977
dc.description.abstractThe distribution of messenger ribonucleic acid (mRNA) containing polyadenylic acid (poly(A)) sequences during the development of the hemipteran, Oncopeltus fasciatus, and the dipteran, Drosophila hydei, was studied in tissue sections by an in situ hybridization method developed as a part of this thesis. The method involves pre-treatment of the sections with DNase followed by annealling of (3H) - polyuridylic acid (poly(U)) to the poly(A) segment of mRNA in tissue sections, digestion of uncomplexed (3H) - poly(U) with ribonuclease, removal of the hydrolysis products by extraction in cold trichloroacetic acid, and visualization of the position of the poly(U):poly(A) hybrids by autoradiography. The poly(U) in situ hybridization method was concluded to be specific for the detection of poly(A) - containing mRNA sequences in tissue sections. The basis for this conclusion was the absence of silver grain development in sections which had been pre-treated with (1) alkali, which is known to destroy RNA but not DNA, (2) pancreatic ribonuclease A in a buffer of low ionic strength, which can completely hydrolyze poly(A) as well as other polyribonucleotides, and (3) T2 RNase, a ribonuclease showing relative specificity for poly(A). [...]
dc.description.departmentBiology and Biochemistry, Department of
dc.format.digitalOriginreformatted digital
dc.format.mimetypeapplication/pdf
dc.identifier.other3329183
dc.identifier.urihttps://hdl.handle.net/10657/9818
dc.language.isoen
dc.rightsThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.
dc.titleAnalysis of the distribution of messenger RNA during insect development by in situ hybridization with radioactive polyuridylic acid
dc.type.dcmiText
dc.type.genreThesis
dcterms.accessRightsThe full text of this item is not available at this time because it contains documents that are presumed to be under copyright and are accessible only to users who have an active CougarNet ID. This item will continue to be made available through interlibrary loan.
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiology, Department of
thesis.degree.disciplineBiology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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