BMP type I receptor inhibition reduces heterotopic ossification
| dc.contributor.author | Yu, Paul B. | |
| dc.contributor.author | Deng, Donna Y. | |
| dc.contributor.author | Lai, Carol S. | |
| dc.contributor.author | Hong, Charles C. | |
| dc.contributor.author | Cuny, Gregory D. | |
| dc.contributor.author | Bouxsein, Mary L. | |
| dc.contributor.author | Hong, Deborah W. | |
| dc.contributor.author | McManus, Patrick M. | |
| dc.contributor.author | Katagiri, Takenobu | |
| dc.contributor.author | Sachidanandan, Chetana | |
| dc.contributor.author | Kamiya, Nobuhiro | |
| dc.contributor.author | Tomokazu, Fukudu | |
| dc.contributor.author | Mishina, Yuji | |
| dc.contributor.author | Peterson, Randall T. | |
| dc.contributor.author | Bloch, Kenneth D. | |
| dc.date.accessioned | 2020-03-10T17:32:08Z | |
| dc.date.available | 2020-03-10T17:32:08Z | |
| dc.date.issued | 2010-03 | |
| dc.description.abstract | Fibrodysplasia ossificans progressiva (FOP) is a congenital disorder of progressive and widespread postnatal ossification of soft tissues1,2,3,4 and is without known effective treatments. Affected individuals harbor conserved mutations in the ACVR1 gene that are thought to cause constitutive activation of the bone morphogenetic protein (BMP) type I receptor, activin receptor-like kinase-2 (ALK2)5. Here we show that intramuscular expression in the mouse of an inducible transgene encoding constitutively active ALK2 (caALK2), resulting from a glutamine to aspartic acid change at amino acid position 207, leads to ectopic endochondral bone formation, joint fusion and functional impairment, thus phenocopying key aspects of human FOP. A selective inhibitor of BMP type I receptor kinases, LDN-193189 (ref. 6), inhibits activation of the BMP signaling effectors SMAD1, SMAD5 and SMAD8 in tissues expressing caALK2 induced by adenovirus specifying Cre (Ad.Cre). This treatment resulted in a reduction in ectopic ossification and functional impairment. In contrast to localized induction of caALK2 by Ad.Cre (which entails inflammation), global postnatal expression of caALK2 (induced without the use of Ad.Cre and thus without inflammation) does not lead to ectopic ossification. However, if in this context an inflammatory stimulus was provided with a control adenovirus, ectopic bone formation was induced. Like LDN-193189, corticosteroid inhibits ossification in Ad.Cre-injected mutant mice, suggesting caALK2 expression and an inflammatory milieu are both required for the development of ectopic ossification in this model. These results support the role of dysregulated ALK2 kinase activity in the pathogenesis of FOP and suggest that small molecule inhibition of BMP type I receptor activity may be useful in treating FOP and heterotopic ossification syndromes associated with excessive BMP signaling. | |
| dc.identifier.citation | Copyright 2008 Nature Medicine. This is a post-print version of a published paper that is available at: https://www.nature.com/articles/nm.1888. Recommended citation:Paul, B. Yu, Donna Y. Deng, Carol S. Lai, Charles C. Hong, Gregory D. Cuny, Mary L. Bouxsein, Deborah W. Hong et al. "BMP type I receptor inhibition reduces heterotopic ossification." Nature medicine 14, no. 12 (2008): 1363. doi:10.1038/nm.1888. This item has been deposited in accordance with publisher copyright and licensing and with the author's permission.. | |
| dc.identifier.uri | https://hdl.handle.net/10657/5974 | |
| dc.language.iso | en_US | |
| dc.publisher | Nature Medicine | |
| dc.title | BMP type I receptor inhibition reduces heterotopic ossification | |
| dc.type | Article |