Bioengineering A Three-Dimensional Cardiac Left Ventricle

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dc.contributor.advisorBirla, Ravi K.
dc.contributor.committeeMemberMay, Elebeoba E.
dc.contributor.committeeMemberLiu, Yu
dc.contributor.committeeMemberMcConnell, Bradley K.
dc.contributor.committeeMemberOmurtag, Ahmet
dc.creatorPatel, Nikita M.
dc.creator.orcid0000-0003-2764-7333
dc.date.accessioned2017-07-21T21:09:27Z
dc.date.available2017-07-21T21:09:27Z
dc.date.createdMay 2015
dc.date.issued2015-05
dc.date.submittedMay 2015
dc.date.updated2017-07-21T21:09:27Z
dc.description.abstractHypoplastic left heart syndrome (HLHS) is a congenital condition characterized by an underdeveloped left ventricle (LV). The current treatment options are surgery and/or heart transplant. Current tissue engineering strategies focus on graft models. The development of an engineered 3D cardiac LV would provide a therapeutic option to overcome current treatment limitations. A series of five models, to understand the ideal LV platform, fabricate and optimize a bioengineered open ventricle chamber and complete the chamber with a trileaflet valve, were produced in this research. Models were designed to emulate the human neonate LV geometry; molds were used to produce chitosan scaffolds. Functional models were fabricated by culturing rat neonatal primary cardiac cells on the chitosan scaffold. Chitosan was shown to be biocompatible with suitable material properties. An open chamber model was designed and optimized with respect to cellularization efficiency and function, using a novel seeding strategy and bioreactor, respectively; cellularized constructs demonstrated cardiac myocyte biopotential activity with contractions and pressure generation. Trileaflet valves were engineered and fitted into the open chamber to complete the bioengineered ventricle. The outcome of this research is the production of a complete bioengineered 3D cardiac LV. 
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.citationPortions of this document appear in: Patel, Nikita M., Ze-Wei Tao, Mohamed A. Mohamed, Matt K. Hogan, Laura Gutierrez, and Ravi K. Birla. "Engineering 3D bio-artificial heart muscle: the acellular ventricular extracellular matrix model." ASAIO journal (American Society for Artificial Internal Organs: 1992) 61, no. 1 (2015): 61. doi: 10.1097/MAT.0000000000000158
dc.identifier.urihttp://hdl.handle.net/10657/1930
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. UH Libraries has secured permission to reproduce any and all previously published materials contained in the work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectTissue Engineering
dc.subjectCardiac
dc.subjectLeft Ventricle
dc.subjectBioengineering
dc.subjectValves
dc.subjectChitosan
dc.subjectThree-dimensional (3D)
dc.titleBioengineering A Three-Dimensional Cardiac Left Ventricle
dc.type.dcmitext
dc.type.genreThesis
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy

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