A MICROFLUIDICS APPROACH TOWARDS THE INVESTIGATION OF FREE HEME EFFECTS ON SICKLE CELL HEMOGLOBIN POLYMERIZATION

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dc.contributor.advisorVekilov, Peter G.
dc.contributor.committeeMemberRimer, Jeffrey D.
dc.contributor.committeeMemberLubchenko, Vassiliy
dc.creatorAich, Anupam
dc.date.accessioned2014-07-25T19:35:27Z
dc.date.available2014-07-25T19:35:27Z
dc.date.createdMay 2012
dc.date.issued2012-05
dc.date.updated2014-07-25T19:35:27Z
dc.description.abstractSickle cell hemoglobin (HbS) polymerization is considered to be the primary pathogenic event in sickle cell anemia. In this work we aim at fabricating an integrated microfluidic device with a localized microheater for the study of HbS polymerization kinetics. We developed fabrication recipes for both an SU-8 based microchannel and a thin film resistive Cu-Cr microheater. We calibrated the heater with both infrared camera and Peltier element and found that a limit exists on the power input to the heater. Computer simulations were carried out to find time resolution of heating process and also the temperature field. Bonding recipe for chip integration has been developed. Surface characterization techniques such as AFM and measuring contact angle with goniometer were used to confirm that oxygen plasma enhances hydrophilicity in SU-8 layer. We found the bonding temperature from differential scanning calorimetry data. Plug flow of liquid in channel has been observed.
dc.description.departmentChemical and Biomolecular Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10657/733
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectMicrofluidics
dc.subjectLozalized microheater
dc.subjectSickle cell disease
dc.subjectHemoglobin
dc.subjectVaso-occlusion
dc.subjectPhotolithography
dc.subjectSU-8
dc.titleA MICROFLUIDICS APPROACH TOWARDS THE INVESTIGATION OF FREE HEME EFFECTS ON SICKLE CELL HEMOGLOBIN POLYMERIZATION
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentChemical and Biomolecular Engineering, Department of
thesis.degree.disciplineChemical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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