Study the Sub-nanometer Membrane Fluctuations in Single Cells Using a Plasmonic Imaging Microscope

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dc.contributor.advisorShan, Xiaonan
dc.contributor.committeeMemberShih, Wei-Chuan
dc.contributor.committeeMemberMayerich, David
dc.contributor.committeeMemberAgrawal, Ashutosh
dc.creatorKhochare, Suraj
dc.date.accessioned2020-01-07T04:00:34Z
dc.date.createdDecember 2018
dc.date.issued2018-12
dc.date.submittedDecember 2018
dc.date.updated2020-01-07T04:00:34Z
dc.description.abstractPlasma membrane of live cells undergo active membrane fluctuations. Scrupulous study of single cell membrane fluctuations can provide better insight into the physiological processes like metabolism, mitosis and cell motility which are very important while developing drug assays for diseases like cancer. Therefore, it is crucial to study the localized membrane fluctuations which pose a technical challenge due to their small yet swift movement. Here, a plasmonic microscope has been used to study membrane fluctuations in HeLa cancer cells. Sub-nanometer level membrane movement has been imaged with a spatial resolution of 0.5 μm in real time. A study of membrane fluctuation amplitude is made to discuss about active membrane movement, cell heterogeneity and interdependence of different cell regions within a single cell. These results could be helpful to study the cell’s mechanoresponse, its viscoelastic properties and analyze the activity of biomarkers when cancer cells are exposed to different drug treatments.
dc.description.departmentElectrical and Computer Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/10657/5837
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectSingle Cancer Cells
dc.subjectPlasmonic Imaging
dc.subjectSub-nanometer Membrane Fluctuations
dc.subjectCell Heterogeneity
dc.subjectCell Metastasis
dc.titleStudy the Sub-nanometer Membrane Fluctuations in Single Cells Using a Plasmonic Imaging Microscope
dc.type.dcmiText
dc.type.genreThesis
local.embargo.lift2020-12-01
local.embargo.terms2020-12-01
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentElectrical and Computer Engineering, Department of
thesis.degree.disciplineElectrical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science in Electrical Engineering

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