The Role of the Drosophila Dopamine 2-Like Receptor in the Blood-Brain Barrier for Male Courtship Behavior
| dc.contributor.advisor | Dauwalder, Brigitte | |
| dc.contributor.committeeMember | Kelleher, Erin S. | |
| dc.contributor.committeeMember | Roman, Gregg | |
| dc.contributor.committeeMember | Lekven, Arne C. | |
| dc.contributor.committeeMember | Bond, Richard A. | |
| dc.creator | Love, Cameron Roger | |
| dc.creator.orcid | 0000-0002-0949-5025 | |
| dc.date.accessioned | 2020-06-04T02:34:21Z | |
| dc.date.created | May 2020 | |
| dc.date.issued | 2020-05 | |
| dc.date.submitted | May 2020 | |
| dc.date.updated | 2020-06-04T02:34:21Z | |
| dc.description.abstract | Courtship in Drosophila Melanogaster is an extensively studied and well characterized complex innate behavior with known molecular determinants. We have previously shown that the G-protein Go-alpha and sex-specific signaling in the Blood-Brain Barrier (BBB) influence male innate courtship sustainment towards naïve females. Here we show that the glial cells that form the BBB modulate male courtship behavior through the dopamine receptor D2R. The subperineurial glia cells (SPG) form the BBB by providing a contiguous barrier, connected by septate junctions, at the interface between the hemolymph and the brain. While the neural circuits required to produce scripted actions in the fly, such as the steps for courtship, have been widely investigated, much less is known about how the circuitry and functions underlying the fly behavior is influenced by cell- non-autonomous molecular processes. We have previously shown that male-specific molecules in the BBB regulate male courtship. We identified the Dopamine-2 like receptor (D2R) RNA as one of a number of sex-specifically enriched BBB transcripts. D2R knockdown with RNAi or over-expression of D2R with a transgene in the SPG of adult males significantly reduces courtship. Knockdown or overexpression of D2R with ubiquitous neuronal drivers or SPG knockdown specifically during development has no effect on the courtship index. D2R mutant flies have courtship defects that can be rescued by expression of wildtype D2R in the BBB of adult males. D2R likely signals through Go and beta-arrestin, to maintain full male courtship levels. Our results indicate an expanded role for dopamine signaling in the glial cells that surround the brain and provide a critical time frame for its action in behavior. | |
| dc.description.department | Biology and Biochemistry, Department of | |
| dc.format.digitalOrigin | born digital | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.citation | Portions of this document appear in: Love, Cameron R., and Brigitte Dauwalder. "Drosophila as a Model to Study the Blood-Brain Barrier." In Blood-Brain Barrier, pp. 175-185. Humana Press, New York, NY, 2019. | |
| dc.identifier.uri | https://hdl.handle.net/10657/6701 | |
| dc.language.iso | eng | |
| dc.rights | The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. UH Libraries has secured permission to reproduce any and all previously published materials contained in the work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s). | |
| dc.subject | Courtship, Dopamine 2-like receptor, Blood-Brain Barrier, Drosophila | |
| dc.title | The Role of the Drosophila Dopamine 2-Like Receptor in the Blood-Brain Barrier for Male Courtship Behavior | |
| dc.type.dcmi | Text | |
| dc.type.genre | Thesis | |
| local.embargo.lift | 2022-05-01 | |
| local.embargo.terms | 2022-05-01 | |
| thesis.degree.college | College of Natural Sciences and Mathematics | |
| thesis.degree.department | Biology and Biochemistry, Department of | |
| thesis.degree.discipline | Biology | |
| thesis.degree.grantor | University of Houston | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | Doctor of Philosophy |
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