Laser Optoacoustic Imaging System for Molecular and Functional Imaging Research in Small Animal Models

dc.contributor.advisorOraevsky, Alexander A.
dc.contributor.advisorLarin, Kirill V.
dc.contributor.committeeMemberGifford, Howard C.
dc.contributor.committeeMemberRoysam, Badrinath
dc.contributor.committeeMemberDas, Mini
dc.contributor.committeeMemberEmelianov, Stanislav Y.
dc.creatorSu, Richard 2021 2021
dc.description.abstractOptoacoustic tomography is an emerging field of medical imaging that is rapidly developing due to its unique capability to visualize and display molecular content of biological tissues with quantitative accuracy and excellent spatial resolution scalable with depth within live tissues. The main merit of optoacoustic tomography is in deep tissue imaging where resolution and contrast of pure optical imaging methods are limited by the strong optical scattering. The dominating tissue chromophores in the spectral range of laser wavelengths (650 nm to 1100 nm) that penetrate deep within tissues are hemoglobin and oxyhemoglobin of blood. Potential capability of functional optoacoustic tomography systems to measure concentrations of hemoglobin and oxyhemoglobin in humans provides for a variety of medical applications in the fields of diagnostics, therapeutic interventions and surgery. Using the methods of molecular imaging, it is also possible to visualize distribution of molecules that do not possess strong optical absorption in the near infrared spectral range, but can be targeted by special molecular and nano-particular contrast agents designed with strong optical absorption and high efficiency of acoustic wave emission through thermal expansion. In spite of great promises the full potential of optoacoustic tomography in functional and molecular imaging has not been realized yet. In order to achieve capabilities of functional and molecular imaging, the optoacoustic tomography system has to overcome the tradeoff between high sensitivity of detection and ultrawide-bandwidth of ultrasonic frequency detection. Furthermore, quantitative imaging is only possible with knowledge of the optical fluence distribution through the entire volume of interest at each of the multiple wavelengths of laser illumination. We took on a challenging task to develop such an advanced tomography system and enhance it with the methods of quantitative data analysis and image reconstruction. We designed and assembled a full view three-dimensional Laser Optoacoustic Imaging System (LOIS-3D) based on a 96-channel array of ultrawide band ultrasonic transducers and demonstrated its highest sensitivity to changes in the optical absorption coefficient ~0.03/cm compared with any academic or industrial system. We proposed and implemented a signal processing method of transducer impulse response deconvolution that enabled reversal of distortions in the detected optoacoustic signals, which in turn allowed the experimental approach to functional and molecular imaging in live laboratory animals. The system technical specifications were characterized in a number of molecular imaging experiments. Finally, we proposed and implemented a practical method of the optical fluence normalization through the entire imaged volume based on measurements of the voxel brightness in blood vessels with known optical absorption and without computations of light propagation through tissues with unknown optical properties. We reconstructed previously unattainable functional images of the total hemoglobin and blood oxygen saturation in a volume of live tissue showing separately arteries, veins and tissues.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.identifier.citationPortions of this document appear in: Su, R., S.A. Ermilov, A.V. Liopo, and A.A. Oraevsky, Three-dimensional optoacoustic imaging as a new noninvasive technique to study long-term biodistribution of optical contrast agents in small animal models. J Biomed Opt, 2012. 17(10): p. 101506.
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. UH Libraries has secured permission to reproduce any and all previously published materials contained in the work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectquantitative imaging
dc.subjectmolecular imaging
dc.subjectfunctional imaging
dc.subjectgold nanorods
dc.subjectindocyanine green
dc.titleLaser Optoacoustic Imaging System for Molecular and Functional Imaging Research in Small Animal Models
local.embargo.terms2023-05-01 College of Engineering Engineering, Department of Engineering of Houston of Philosophy


Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
3.9 MB
Adobe Portable Document Format

License bundle

Now showing 1 - 2 of 2
No Thumbnail Available
4.43 KB
Plain Text
No Thumbnail Available
1.81 KB
Plain Text