Coaxial Electrospinning of Protein-Encapsulated Core-Shell Nanofibers: Process Optimization and Release Modeling

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dc.contributor.advisorAbidian, Mohammad Reza
dc.contributor.committeeMemberMohan, Chandra
dc.contributor.committeeMemberMajd, Sheereen
dc.creatorHariprasad, Bhoomija
dc.creator.orcid0000-0003-0279-1670
dc.date.accessioned2020-01-03T05:44:43Z
dc.date.createdDecember 2019
dc.date.issued2019-12
dc.date.submittedDecember 2019
dc.date.updated2020-01-03T05:44:43Z
dc.description.abstractCoaxial electrospinning is a novel method for encapsulation of protein drugs into polymeric materials for use in drug delivery systems. In this study, coaxial electrospinning was used to fabricate aligned polyethylene oxide/poly(lactic-co-glycolic acid) core-shell nanofibers encapsulated with nerve growth factor (NGF), a trophic agent for axonal regeneration. Electrospinning processing parameters, namely inner and outer flow rates, wheel speed, needle-wheel distance, and applied voltage, were optimized using design of experiment (DOE) methodology to achieve nanofibers with minimized diameter and size distribution. The resulting prediction models were validated using analysis of variance. Optimized fibers were incubated in phosphate-buffered saline (PBS) for 3 days, and the released NGF was characterized at different time points using ELISA. The NGF release profile was mathematically modeled utilizing the Korsmeyer-Peppas and zero-order models. The results of this study can be applied to drug delivery systems for neural regeneration.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/10657/5650
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectCoaxial electrospinning
dc.subjectDesign of Experiment optimization
dc.titleCoaxial Electrospinning of Protein-Encapsulated Core-Shell Nanofibers: Process Optimization and Release Modeling
dc.type.dcmiText
dc.type.genreThesis
local.embargo.lift2021-12-01
local.embargo.terms2021-12-01
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science in Biomedical Engineering

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