Inflammation in Zebrafish Models
| dc.contributor.advisor | Mohan, Chandra | |
| dc.contributor.committeeMember | Wu, Tianfu | |
| dc.contributor.committeeMember | Bondesson, Maria | |
| dc.contributor.committeeMember | Pathak, Simanta | |
| dc.creator | Zambrano Cobo, Amarayca | |
| dc.date.accessioned | 2019-09-18T19:03:34Z | |
| dc.date.available | 2019-09-18T19:03:34Z | |
| dc.date.created | May 2015 | |
| dc.date.issued | 2015-05 | |
| dc.date.submitted | May 2015 | |
| dc.date.updated | 2019-09-18T19:03:34Z | |
| dc.description.abstract | The zebrafish is an outstanding model for in-vivo imaging of inflammation due to its optical translucency and the ability of transgenic lines to express fluorescent proteins under specific promoters. Furthermore, the immune system of zebrafish closely resembles that of mammals. Inflammation is the body's attempt at self-protection; the aim being to remove harmful stimuli, including damaged cells, irritants, or pathogens - and begin the healing process. This study focuses on two zebrafish models of inflammation. The first model, Tag MPX:GFP (UWM1), which has already been established and studied, was used to investigate the possible effect of Epigallocatechin gallate (EGCG) in the inflammation process. The second model will express mCherry fluorescent protein under the Mx promoter, in order to quantify the strength or activity of upstream gene expression. The results suggest that EGCG has a favorable effect on the inflammation process. In addition, the entry clone for the Mx1-mCherry system was successfully constructed. | |
| dc.description.department | Biomedical Engineering, Department of | |
| dc.format.digitalOrigin | born digital | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://hdl.handle.net/10657/4848 | |
| dc.language.iso | eng | |
| dc.rights | The author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s). | |
| dc.subject | Zebrafish | |
| dc.subject | Inflammation | |
| dc.title | Inflammation in Zebrafish Models | |
| dc.type.dcmi | Text | |
| dc.type.genre | Thesis | |
| thesis.degree.college | Cullen College of Engineering | |
| thesis.degree.department | Biomedical Engineering, Department of | |
| thesis.degree.discipline | Biomedical Engineering | |
| thesis.degree.grantor | University of Houston | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science |