Tissue Engineered Heart Pump Development and Assessment

dc.contributor.advisorBirla, Ravi K.
dc.contributor.committeeMemberSchwartz, Robert J.
dc.contributor.committeeMemberMay, Elebeoba E.
dc.contributor.committeeMemberMcConnell, Bradley K.
dc.contributor.committeeMemberOmurtag, Ahmet
dc.creatorMohamed, Mohamed Ahmed
dc.date.createdMay 2016
dc.date.submittedMay 2016
dc.description.abstractDevelopment of a natural alternative to cardiac assist devices (CADs) will pave the way to a heart failure therapy which overcomes the disadvantages of current mechanical devices. Through implementation of the three principles of tissue engineering, cell sourcing, material scaffolding, and bioreactors, development of a tissue engineered heart pump (TEHP) can be a viable biological CAD option. An experimental model of a TEHP was first fabricated by wrapping artificial heart muscle (AHM), composed of rat neonatal cardiac cells on the surface of a fibrin gel, around an acellular goat carotid artery (GCA) and a chitosan hollow cylinder (CHC) scaffold in various configurations. Histological assessments revealed the presence of cardiac cell layer cohesion and adhesion, as well as retention of cardiac myocyte phenotype. Biopotential measurements revealed the presence of ~2.5 Hz rhythmic propagation of action potential throughout the TEHP. A more clinically applicable TEHP was then fabricated by use of human adipose derived mesenchymal cells (hADMCs), which have been programmed towards a cardiac lineage, in conjunction with a chitosan scaffold imbued with purified porcine extracellular matrix proteins. The second generation TEHP was lined with human endothelial cells and conditioned with pulsatile flow and electrical stimulus. As a result, hADMCs were further matured along their cardiac potential and the TEHPs they embodied formed the foundation for biological CADs.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.identifier.citationPortions of this document appear in: Tao, Ze-Wei, Mohamed Mohamed, Matthew Hogan, Betsy Salazar, Nikita M. Patel, and Ravi K. Birla. "Establishing the framework for fabrication of a bioartificial heart." Asaio Journal 61, no. 4 (2015): 429-436. doi: 10.1097/MAT.0000000000000233; and in: Tao, Ze‐Wei, Mohamed Mohamed, Matthew Hogan, Laura Gutierrez, and Ravi K. Birla. "Optimizing a spontaneously contracting heart tissue patch with rat neonatal cardiac cells on fibrin gel." Journal of tissue engineering and regenerative medicine 11, no. 1 (2017): 153-163. DOI: 10.1002/term.1895.
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. UH Libraries has secured permission to reproduce any and all previously published materials contained in the work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectTissue Engineering
dc.titleTissue Engineered Heart Pump Development and Assessment
thesis.degree.collegeCollege of Natural Sciences and Mathematics
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.nameDoctor of Philosophy


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