Optimization of PEGDA Hydrogel Platform for Gel Electrophoresis Application

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dc.contributor.advisorAkay, Metin
dc.contributor.committeeMemberAkay, Yasemin M.
dc.contributor.committeeMemberWu, Tianfu
dc.creatorCandelari, Christopher
dc.creator.orcid0000-0003-3772-0637
dc.date.accessioned2017-07-17T16:15:18Z
dc.date.available2017-07-17T16:15:18Z
dc.date.createdMay 2017
dc.date.issued2017-05
dc.date.submittedMay 2017
dc.date.updated2017-07-17T16:15:18Z
dc.description.abstractWe have developed a micrometer scale thick electrophoresis system based on polyethylene glycol dimethylacrylate (PEGDA, Mn = 750) to separate nucleic acids. Using photo-polymerization, we fabricated PEGDA hydrogel attached to cover glass that is robust, easily handled and comparable to agarose gels in DNA band detection. Optimization parameters including PEGDA concentration, UV exposure time, voltage, run-time and hydrogel thickness were determined and optimized for PEGDA-750 to enable the separation of standard DNA ladder bands ranging from 250 to 1000 base pairs. Our optimized method for creating PEGDA hydrogels is capable of performing DNA electrophoresis and holds promise for being further developed as a platform for detecting specific ranges of nucleic acids. This study enhanced parameters surrounding DNA band separation and brightness, and refined fabrication methods. PEDGA hydrogels hold potential to be a highly customizable alternative to agarose gels because of their distinct photo- polymerization fabrication method and attachment to cover glass.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10657/1887
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectGel electrophoresis
dc.subjectHydrogel
dc.subjectPEG
dc.subjectPEGDA
dc.subjectPolyethylene glycol
dc.subjectPhoto-polymerization
dc.subjectCross-linking
dc.subjectAgarose
dc.subjectGel
dc.titleOptimization of PEGDA Hydrogel Platform for Gel Electrophoresis Application
dc.type.dcmitext
dc.type.genreThesis
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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