The Endogenous Metabolite 5-Methoxytryptophan as a Potential Novel Therapy for Pancreatic Cancer

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dc.contributor.advisorRuan, Ke-He
dc.contributor.committeeMemberDixon, Richard A. F.
dc.contributor.committeeMemberWilliams, Louis
dc.contributor.committeeMemberDas, Joydip
dc.contributor.committeeMemberGao, Xiaolian
dc.creatorMurdoch, Emma E.
dc.creator.orcid0000-0003-3638-0837
dc.date.accessioned2018-03-01T17:54:19Z
dc.date.available2018-03-01T17:54:19Z
dc.date.createdAugust 2017
dc.date.issued2017-08
dc.date.submittedAugust 2017
dc.date.updated2018-03-01T17:54:19Z
dc.description.abstractPancreatic adenocarcinoma is a highly aggressive cancer, and due to a lack of early detection methods, is often diagnosed in the late stages of the disease. One reason for the poor prognosis in pancreatic cancer is the repopulation of tumors, which is driven by pathological cyclooxygenase-2 production. The L-tryptophan metabolite 5-MTP has previously been shown to reduce COX-2 transcription via the p300 histone acetyltransferase. In a scratch test assay using the PANC-28 cell line, we demonstrated that PANC-28 cell invasion could be reduced by treatment with 5-MTP at the micromolar level. This project included molecular modeling of 5-MTP at the PGE2 receptor EP3. 5-MTP showed a strong binding score, in a similar location to that of the endogenous prostaglandins. A cell counting kit was used to measure cell survival following 5-MTP administration, and showed that 5-MTP was non-toxic to cells. Given our results, it is possible that 5-MTP may have a beneficial role in pancreatic cancer. Future studies will include biochemical and ligand binding assays to build upon these findings.
dc.description.departmentPharmacological and Pharmaceutical Sciences, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10657/2658
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subject5-methoxytryptophan
dc.subjectCyclooxygenase-2
dc.subjectProstaglandin E2 (PGE2)
dc.subjectProstaglandin receptors
dc.titleThe Endogenous Metabolite 5-Methoxytryptophan as a Potential Novel Therapy for Pancreatic Cancer
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCollege of Pharmacy
thesis.degree.departmentPharmacological and Pharmaceutical Sciences, Department of
thesis.degree.disciplinePharmacology
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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