Engineering Alkane-Inducible Fimbriation in Escherichia Coli

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dc.contributor.advisorConrad, Jacinta C.
dc.contributor.advisorCirino, Patrick C.
dc.contributor.committeeMemberRodrigues, Debora F.
dc.creatorMcLay, Ryan B.
dc.date.accessioned2018-07-10T18:51:13Z
dc.date.available2018-07-10T18:51:13Z
dc.date.createdMay 2016
dc.date.issued2016-05
dc.date.submittedMay 2016
dc.date.updated2018-07-10T18:51:13Z
dc.description.abstractFimbriae are small, hair-like appendages arranged on many bacteria’s outer-membrane surfaces used for binding to biotic and abiotic surfaces to resist shear forces and attach to nutrient sources. Fimbriae are important in the initial attachment of cells to a substrate as well as the subsequent irreversible attachment to a surface. In this investigation, E. coli was engineered to over-express fimbriae in response to the presence of IPTG and DCPK. The over-expression of fimbriae in E. coli resulted in a pronounced response to the agglutination of mannose-containing yeast cells, causing them to sediment from solution at a much faster rate than wild-type or fimbrial deletion strains. Furthermore, the presence of fimbriae resulted in a significantly higher partitioning of cells into hydrocarbon sources across a range of alkanes. Lastly, the presence of fimbriae was shown to be essential in the development of biofilms, where only strains expressing fimbriae resulted in biofilm formation.
dc.description.departmentChemical and Biomolecular Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10657/3205
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectEscherichia coli
dc.subjectFimbriae/pili
dc.subjectIPTG-inducible
dc.subjectDCPK-inducible
dc.subjectHydrocarbons
dc.subjectAlkanes
dc.subjectMicrobial adhesion to hydrocarbons (MATH)
dc.subjectSpecific biofilm formation (SBF)
dc.subjectYeast agglutination assay (YAA)
dc.subjectBiofilms
dc.subjectBioremediation
dc.subjectFimbriation
dc.titleEngineering Alkane-Inducible Fimbriation in Escherichia Coli
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentChemical and Biomolecular Engineering, Department of
thesis.degree.disciplineChemical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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