Bedside Washing of Stored Red Blood Cells: A Simple Apparatus Based on Microscale Sedimentation in Normal Gravity

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dc.contributor.advisorShevkoplyas, Sergey S.
dc.contributor.committeeMemberMohan, Chandra
dc.contributor.committeeMemberGeorge, Alex
dc.contributor.committeeMemberWu, Tianfu
dc.creatorHuynh, Rose Ann A.
dc.creator.orcid0000-0002-8260-5104
dc.date.accessioned2020-01-03T23:14:23Z
dc.date.createdMay 2015
dc.date.issued2015-05
dc.date.submittedMay 2015
dc.date.updated2020-01-03T23:14:24Z
dc.description.abstractThe aim of this study was to develop and evaluate a simple, inexpensive bedside RBC washer with the capability to process multiple pRBC units within a short period without any alteration to current blood collection and storage practices. Washing pRBC units prior to transfusion is recommended for hypersensitive patients who have a higher susceptibility towards Allergic Transfusion Reactions (ATR) due to residual plasma proteins. Unfortunately, conventional washing is bulky, expensive and laborious, due to concentrating washed units through centrifugation. In response, we developed a bedside RBC washer that utilizes gravitational sedimentation of RBCs within a saline suspension. We evaluated its ability to concentrate washed pRBC units to a therapeutic hematocrit of 65%, but it optimally concentrated to a physiological hematocrit of 41%. Concentration was found to be dependent on initial morphology of the unit. In terms of improved quality, the gravity-driven washing system performed comparably with conventional washing.
dc.description.departmentBiomedical Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttps://hdl.handle.net/10657/5690
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectBedside Washing
dc.subjectStored Red Blood Cells
dc.titleBedside Washing of Stored Red Blood Cells: A Simple Apparatus Based on Microscale Sedimentation in Normal Gravity
dc.type.dcmiText
dc.type.genreThesis
local.embargo.lift2020-05-01
local.embargo.terms2020-05-01
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentBiomedical Engineering, Department of
thesis.degree.disciplineBiomedical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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