Quantification and Molecular Characterization of Anti-citrulline Antibodies in Rheumatoid Arthritis

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dc.contributor.advisorVaradarajan, Navin
dc.contributor.committeeMemberKrishnamoorti, Ramanan
dc.contributor.committeeMemberEconomou, Demetre J.
dc.creatorLie, Anthony 1987-
dc.date.accessioned2018-02-15T20:10:13Z
dc.date.available2018-02-15T20:10:13Z
dc.date.createdAugust 2012
dc.date.issued2012-08
dc.date.submittedAugust 2012
dc.date.updated2018-02-15T20:10:13Z
dc.description.abstractAutoimmune diseases are believed to result from the inability of the immune system to distinguish self and non-self. Rheumatoid Arthritis (RA) is a systemic autoimmune disease that leads to joint destruction and affects more than 1.5 million adults (Centre for Disease Control). Auto-antibodies against citrullinated proteins (ACPA) are present in approximately 60-75% of RA patients with 96% specificity. Although the importance of ACPA, both as a causative agent and diagnostic marker, has been established, the reactivities of single ACPA and the eptiopes are unknown. Isolation and characterization of protein targets of ACPA would shed light on the underlying mechanism of RA and would offer (i) earlier diagnosis and (ii) routes for therapeutic intervention. Here, we employ a novel high-throughput methodology, microengraving, based on fabricated nanowells arrays, to isolate antibodies from stimulated memory B cells of RA patients. In conjunction with single-cell RT-PCR amplification this technique is employed to perform molecular characterization of ACPA antibodies.
dc.description.departmentChemical and Biomolecular Engineering, Department of
dc.format.digitalOriginborn digital
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/10657/2194
dc.language.isoeng
dc.rightsThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).
dc.subjectRheumatoid Arthritis
dc.subjectACPA
dc.subjectAuto-antibodies
dc.subjectCCP
dc.subjectNanowell arrays
dc.subjectSingle cell
dc.titleQuantification and Molecular Characterization of Anti-citrulline Antibodies in Rheumatoid Arthritis
dc.type.dcmiText
dc.type.genreThesis
thesis.degree.collegeCullen College of Engineering
thesis.degree.departmentChemical and Biomolecular Engineering, Department of
thesis.degree.disciplineChemical Engineering
thesis.degree.grantorUniversity of Houston
thesis.degree.levelMasters
thesis.degree.nameMaster of Science

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