Browsing by Author "Wang, Mengfan"
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Item Development Of A High-Throughput Flow Biofilm Reactor System For The Study of Bacterial Interference Against Uropathogenic Colonization On Silicone Urinary Catheters(2019) Thang, Christopher; Wang, Mengfan; Cashin, ThomasIn this project, a 34-channel catheter array system was designed to investigate our bacterial interference strategy under in vivo flow conditions. The catheter model system consists of three main parts: an ultraviolet (UV) disinfection subsystem; a 100L artificial urine preparation, storage, and flow guiding subsystem; and a 34-channel catheter array. This catheter-model project opens vast possibilities toward studying the interactions among nonpathogenic and pathogenic bacteria involved in CAUTI and its prevention based on the bacterial interference strategy.Item Effect of Catheter Surface Mannosylation on Fim+ E. coli 83972 Biofilm Formation and Prevention of Uropathogenic Colonization(2023-08) Wang, Mengfan; Cai, Chengzhi; Chen, Tai-Yen; Walker, Jennifer N.; Comito, Robert J.; Do, Loi H.Catheter‑associated urinary tract infections (CAUTIs) are the most common health care‑associated infection. It is greatly facilitated by the formation of pathogenic bacterial biofilms on urinary catheters. In this work, we aimed to form stable and dense non-pathogenic biofilm by fim+ E. coli 83972 on modified catheter surfaces, and use the preformed biofilm to prevent the colonization of pathogens. It is well known that the extracellular polymeric substances (EPS) secreted by bacteria play a critical role on biofilm formation and stability. While majority of the EPS study focuses on polysaccharides, little is known about the composition and function of the EPS proteome. In this project, we modified urinary catheter surfaces with a series of mannosides with a wide range of binding affinities to the type 1 fimbriae on fim+ E. coli 83972, and investigated how the mannoside binding affinities affects the biofilm formation, stability and bacterial interference by this non-pathogenic bacteria against uropathogens. We also aimed to gain molecular level understanding of the effect of immobilized mannosides on the EPS proteome secreted by the bacteria during early stage biofilm formation. For the first time, we developed a workflow to extract as little as 20 ng of EPS proteins during the early stage (0.5 - 4 h) of biofilm development. Using LC-MS/MS, we identified more than 1500 EPS proteins. Significantly, 40% of the common EPS proteins found in all four mannoside coated surfaces were not characterized in PATHER categories. The time-course profile of the abundance each protein was obtained and cluster analysis was performed to provide new insights into the protein expression of fim+ E. coli+ 83972 biofilm formation. Moreover, a high-throughput flow catheter array system was developed to systematically study the biofilm formation under (intermittent) flow conditions resembling the clinical settings. Finally, we successfully reduced the colonization of pathogenic bacteria (E. coli 3152) by over 1000 times by preforming fim+ E. coli 83972 biofilm on BiPh-CF3 PAMAM coated catheters.