Browsing by Author "Rodgers, Alden Van Vechten"
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Item A preliminary investigation of the myogens of adult commercial shrimp(1964) Rodgers, Alden Van Vechten; Bartel, Allen H.; Kimball, Aubrey P.; Evans, John E.; Aumann, Glenn DavidA preliminary study was made of myogens from juvenile and adult shrimp, Penaeus setiferus Linnaeus ("white"), P. Aztecus Ives ("brown") and P. duoranum Burkenroad ("pink") to determine whether the electrophoretic behavior of these proteins can be used to identify these species. When examined by acrylamide gel and cellulose-acetate electrophoresis, it was found that these species can be identified by the electrophoretic behavior of their myogens. The sizes of white and brown shrimp myogens are similar as shown by their chromatographic behavior on Sephadex G-75. On the basis of calibration of the gel with known proteins, the three peaks observed were estimated to consist of molecules of molecular weight greater than 69,000, 30,000, and less than 3,000 daltons. The peak with low molecular weight components contained nucleotides, nucleosides, and ninhydrin positive molecules. Two nucleotides were identified tentatively as 5' IMP (inosine-5'-monophosphate) and 5' CMP (cytidine-5'-monophosphate) and one nucleoside as inosine. One of the ninhydrin positive molecules has been identified tentatively as proline or hydroxyproline.