Browsing by Author "Daugherty, Caleb M."
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Item Investigation of the Effect of Crowding on Cooperativity in the Folding of Phosphoglycerate Kinase(2017-10-12) Daugherty, Caleb M.; Gasic, Andrei G.Experiments on the folding mechanics of Phosphoglycerate Kinase(PGK) confirm a two state thermal transition between the native and denatured states, and a multi-state transition under pressure denaturation. -The introduction of crowding agents reduces the intermediate states under pressure denaturation. -Classical folding theory is based on in vitro experimentation, but does not accurately predict folding mechanics in the crowded cellular environment. -Understanding the in vivo folding process may lead to better treatments for disease associated with protein misfolding.