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dc.contributorSen, Mehmet
dc.contributor.authorKoelbel, Skylar
dc.date.accessioned2018-02-27T15:51:50Z
dc.date.available2018-02-27T15:51:50Z
dc.date.issued2017
dc.identifier.urihttp://hdl.handle.net/10657/2503
dc.description.abstractROCK1 is a kinase involved in regulating actin-myosin contraction, smooth muscle contraction, morphology, and cytoskeleton organization. Purpose: Optimization of expression will allow us to produce a homogenous Rock1 protein, which is absolutely required for protein crystallization as well as drug discovery studies in our lab. Conclusions. ROCK1 protein was only expressed in LONZA cell media. In LONZA, protein expression was best at 0.25 to 0.5 MOI. Protein expression is selective, suggesting conditions such as different cell media should be taken into account. Viral infection is best at a lower MOI so the virus does not kill the cells before the protein can be expressed.
dc.language.isoen_US
dc.relation.ispartofSummer Undergraduate Research Fellowship
dc.titleOptimization of ROCK1 Protein Through Insect Cells
dc.typePoster
dc.description.departmentBiology and Biochemistry
dc.description.departmentHonors College


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