Verification of Chromosomal Inversions along the tgrB1-C1 loci in Dictyostelium discoideum
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Chromosomal inversions readily occur in nature, but their effects have not been thoroughly studied. Dictyostelium discoideum is a social amoeba, that when depleted of food, aggregate into multicellular fruiting bodies consisting of viable spores and stalks made of dead cells. However, when genetically dissimilar cells co-aggregate, some strains can “cheat” other populations into donating a larger portion to the stalk. Kin-recognition is strongly mediated by different alleles of the tgrB1-tgrC1 loci that belong to a large gene family named tiger. 5 wild-types did show positive results upon gel electrophoresis but sequencing determined the results were due to non-specific binding. Sequencing revealed large portions of AT-rich regions that could account for non-specificity during PCR.