Investigation of the Effect of Crowding on Cooperativity in the Folding of Phosphoglycerate Kinase
Daugherty, Caleb M.
Gasic, Andrei G.
MetadataShow full item record
Experiments on the folding mechanics of Phosphoglycerate Kinase(PGK) confirm a two state thermal transition between the native and denatured states, and a multi-state transition under pressure denaturation. -The introduction of crowding agents reduces the intermediate states under pressure denaturation. -Classical folding theory is based on in vitro experimentation, but does not accurately predict folding mechanics in the crowded cellular environment. -Understanding the in vivo folding process may lead to better treatments for disease associated with protein misfolding.