Transcriptome Analysis of Colchicine Producing Plant Species: Gloriosa superba and Colchicum autumnale
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Colchicine is an alkaloid product of plant biosynthesis which is FDA approved and commonly used to treat gout. It has also been proven to be beneficial in heart attack recovery and its antimitotic properties make it a promising cancer treatment. Traditionally, the natural isomer of colchicine is exacted from Gloriosa superba after harvesting the plant and the compound itself was initially isolated from Colchicum autumnale. The biosynthetic pathway of colchicine is not yet characterized, so in order to improve colchicine production in manufacturing, the pathway genes and mechanisms must be researched and clarified. To elucidate this biosynthetic pathway G. superba and C. autumnale transcriptomes were analyzed and compared against NCBI and Swissprot protein databases. Afterwards, annotation data of these transcriptomes revealed that there were 60927 assembled multi-tissue transcripts of C. autumnale which represented 21948 unigenes and G. superba has 32312 assembled multi-tissue transcripts which represented 15089 unigenes in known plantspecific Gene Ontology (GO). In order to further identify colchicine pathways and gene, further GO term analysis was performed using the Kyoto Encyclopedia of Genes and Genomes (KEGG) for both organisms. The results obtained represents comparative GO term and KEGG pathways, which could aid in identifying colchicine pathway genes.