2022-06-202022-06-2019763826859https://hdl.handle.net/10657/9472he cytological studies of Vicia fata, Luzula purpurea, and L. multiflora and the biophysical characterization of L. purpurea DNA revealed several interesting features to occur in both the organisms and DNA of organisms lacking localized centromeres. Vicia faba, an organism having chromosomes with localized centromeres, exhibited a very restricted nuclear and chromosomal distribution for C-positive, Q-positive, and DNA late-replicating regions. These regions were concentrated in the vicinity of the centromeres. Multiple C-positive and DNA late-replicating regions were scattered throughout both the nuclei and chromosomes of L. purpurea and L. multiflora. The C-positive, DNA late-replicating chromosome regions of L. purpurea were also Q-positive and composed of highly repeated DNA sequences as revealed by ^3H-RNA in situ hybridization experiments. The different techniques permitted recognition of each of the three chromosome pairs of L. purpurea even though the chromosomes are equal-sized and lack primary constrictions. [...]application/pdfenThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.Thesisreformatted digital