Evans, John E.2022-07-072022-07-07197123155148https://hdl.handle.net/10657/10448Synchronous division of Escherichia coli K12 Hfr Ca 3000 met- thi- thy- in a complex medium has been obtained using stationary- phase synchrony. As many as five synchronous cell cycles have been followed using a 1:60 dilution. It was also shown that balanced growth was obtained in at least three cell cycles. Decreasing the inoculum size resulted in a shorter lag, less variation in the generation times and a higher final synchronous cell density up to an optimum dilution. The concept of an inflection point has been defined, and its relationship to final synchronous cell density has been established. The phenomenon of a division clock in bacteria has been investigated.application/pdfenThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.Thesisreformatted digital