Becker, Ralph S.2022-08-102022-08-101966196817885685https://hdl.handle.net/10657/10754The importance of DNA-protein-small molecule interactions in the control of genetic expression is now clearly established. Spectrophotometric and fluorometric titration of acridine orange, 9-aminoacridine, and ethidium bromide with DNA and a model nucleoprotein, composed of DNA complexed with poly-L-lysine, indicate that these small molecules successfully compete with poly-L-lysine for binding sites on DNA. A comparison of the absorption spectra of l-hydroxyacridine and l-hydroxyanthracene in the presence of DNA gives some insight into the nature of acridine-DNA interaction under the conditions of our experiments. Also described are preliminary efforts toward the extension of existing techniques to the study of carcinogenic hydrocarbons in this context. The possible significance in nature of small molecule-protein competition for DNA binding sites is discussed.application/pdfenThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.Thesisreformatted digital