Rodrigues, Debora F.2017-08-162017-08-16May 20152015-05May 2015Portions of this document appear in: Okyay, Tugba Onal, and Debora Frigi Rodrigues. "Optimized carbonate micro-particle production by Sporosarcina pasteurii using response surface methodology." Ecological engineering 62 (2014): 168-174. https://doi.org/10.1016/j.ecoleng.2013.10.024. And in: Okyay, Tugba Onal, and Debora F. Rodrigues. "Biotic and abiotic effects on CO2 sequestration during microbially-induced calcium carbonate precipitation." FEMS microbiology ecology 91, no. 3 (2015). https://doi.org/10.1093/femsec/fiv017. And in: Okyay, Tugba Onal, and Debora Frigi Rodrigues. "High throughput colorimetric assay for rapid urease activity quantification." Journal of microbiological methods 95, no. 3 (2013): 324-326. https://doi.org/10.1016/j.mimet.2013.09.018.http://hdl.handle.net/10657/2021The development of affordable and eco-friendly strategies for carbon dioxide sequestration has become a matter of paramount importance to reduce or mitigate the effects of global climate changes. Today, the most used solution to sequester CO2 is its immobilization in geological reservoirs, commonly referred to as carbon capture and storage; however this technique is not completely reliable because of leakage risks, when storing vast quantities of CO2 in geological strata. Alternatively, precipitation of CO2 as solid carbonates may constitute an alternative strategy for carbon immobilization. The reaction to form calcium carbonates is generally not chemically favorable in the environment, unless at pH values higher than 9. On the other hand, microorganisms, through metabolic activities, have been shown to induce calcium carbonate precipitation, provided that certain environmental conditions are met. In this dissertation, I investigate the diversity and physiology of diverse ureolytic consortia and isolates able to induce calcium carbonate precipitation to better understand their roles in carbon sequestration. These microorganisms were obtained from karstic environments that are rich in calcium and present natural input of urea, which are considered to be key factors in calcium carbonate precipitation. These urease-positive microorganisms were classified phylogenetically and their physiology was investigated. The relationship amongst urease activity, microbially-induced calcium carbonate precipitation (MICP), and carbon sequestration by the different consortia and isolates were shown to be dependent on the species and directly influenced by their growth conditions.application/pdfengThe author of this work is the copyright owner. UH Libraries and the Texas Digital Library have their permission to store and provide access to this work. UH Libraries has secured permission to reproduce any and all previously published materials contained in the work. Further transmission, reproduction, or presentation of this work is prohibited except with permission of the author(s).Calcium carbonatePrecipitationCO2 sequestrationBacterial isolatesBacterial consortiaCaveTravertineSporosarcina pasteurii2017-08-16Thesisborn digital