Kimball, Aubrey P.2024-04-222024-04-22198311382669https://hdl.handle.net/10657/17038The combination of 2'-deoxyadenosine and deoxycoformycin is known to be markedly toxic to T-lymphocyte cell lines relative to B-cell lines and this difference appears to be related to the capacity of the cells to accumulate dATP. In the presence of dipyridamole and 2'-deoxyadenosine and when adenosine deaminase was inhibited with deoxycoformycin, the L1210 leukemia cell which is a non-T, non-B cell type accumulated dATP like a T-cell type. The intracellular L1210 dATP levels using the triple combination (1.1 [micrometers] deoxycoformycin, 40 [micrometers] deoxyadenosine, 10 [micrometers] dipyridamole) reached 360 nmol/10[^9] cells at which concentration ribonucleotide reductase specific activity was reduced by 80%. In addition to the increase in dATP levels, intracellular L1210 2'-deoxyadenosine levels as high as 375 nmol/10[^9] cells were obtained with the triple combination.application/pdfenThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. Section 107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.DeoxyadenosineB cellsT cellsThesisreformatted digital