Bartel, Allen H.2022-05-192022-05-1919752752390https://hdl.handle.net/10657/9128Phenylalanine hydroxylase from dog liver was purified ten fold. The physical and kinetic properties of phenylalanine hydroxylase from dog liver in many respects closely resemble physical and kinetic properties of the same enzyme from other organism reported in the literature. The Km value determined for phenylalanine hydroxylase from dog liver for phenylalanine is 3.7 [plus-minus] 1.1 mN; for 6,7-dimethyltetrahydropterin is 0.17 [plus-minus] 0.10 mM; and for tetrahydrobiopterin is 0.34 [plus-minus] 0.03 mH. A new assay developed in this study is especially suited for detection of phenylalanine hydroxylase from dog liver and it makes possible the detection of enzyme activity at low concentration of enzyme.application/pdfenThis item is protected by copyright but is made available here under a claim of fair use (17 U.S.C. §107) for non-profit research and educational purposes. Users of this work assume the responsibility for determining copyright status prior to reusing, publishing, or reproducing this item for purposes other than what is allowed by fair use or other copyright exemptions. Any reuse of this item in excess of fair use or other copyright exemptions requires express permission of the copyright holder.Thesisreformatted digital